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Abstract In this study we made Isolation and identification of S. aureus from baby chicks by taking 300specimens from 100 diseased chicks from different Egyptian govemorates, including (Dakahleia, Damietta, Kafr el-Sheikh and Gharbia), made isolation and identification , sensitivity test and Per Jest to the coagulase positive Scaureus . By bacteriological examination of these specimens we found that a total of 139 out of 300 with percentage of 46.3% were found positive for Staph. species and 161 out of 300 with percentage of 53.6% were negative for Staph. species and atotal of 26 specimens of l39 with percentage 18.7% were found coagulase positive and 113 specimens of l39 with percentage of 81.2% were found coagulase negative. The isolated coagulase positive S.aureus of diseased chicks from different was from Liver, yolk sac, and heart with percentages of 40.3 %,33.9%,25.8 %, respectively. Examination of 26 samples of coagulase positive S.aureus against 8 antimicrobial discs revealed different percentage of sensitivity as it illustrated in Table (7) and. In regard to Oxacillin 21 coagulase positive S.aureus was resistant (80.76%) while, 5 strains were intermediate (19.23%). On the other hand 3 (1l.53%) of the strains were resistant and 23 (88.46%) sensitive to vancomycin. 7 (26.92%) of the strains were resistant while, 19 (73.07%) were sensitive to Gentamycin. But 1 (3.84%) and 25 (96.15%) of the strains were resistant and sensitive to ciprofloxacin, respectively. On the other hands,15 (57.69%) of the strains were resistant, while, 2 |