الفهرس | Only 14 pages are availabe for public view |
Abstract Microsporum canis is zoophilic dermatophytes usually cause ringworm in dogs and cats. It has been frequently isolated from human cases of tinea capitis and tinea corporis. The present work dealt with the methods of phenotypic and genotypic identification of M. Canis isolated from human, dogs and cats. from 50 samples of skin scrapings and hair collected from human dermatophytosis (30 tinea capitis and 20 tinea corporis) 29 isolates (58%) were suspected as M. canis, while from 21 samples collected from dogs and cats suffering from ringworm, 18 isolates (85.71%) of M. canis were obtained. Forty seven isolates suspected as M. canis by characters on SDA and DTM were subjected for identification through phenotypic identification methods (macro-and micromorphology and culture on differential medium) and genotypic methods (PCR for ITS and Sequences ). Macro-morphology of all isolates showed cottony growth with dark yellow color reverse, while micro-morphological examination revealed the presence of large spindle thick walled macroconidia with presence of hook or knob at its end and clavated microconidia. Some isolates demonstrated pleomorphism and absence of conidia. Cultivation on differential media as a method for identification of dennatophytes, M canis isolates revealed profuse growth without change on colour of BCP medium, profuse growth with yellow diffuse pigment on LRA medium and fluffy to wooly colonies with yellow. |