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Abstract Some ”toxicological studies on fusariotoxins in dairy ruminants were obtained in this investigation, the studies include: 1- The first part aimed to study the prevalence and the population density of fungi, screening of Fusarium species isolated for the production of toxins (zearalenone and zearalenol), and the natural occurrence of different mycotoxins and also their toxicity in twenty feed samples collected from the milk production farms and Bani-Korra oil and commercial concentrate mixture factory in Assiut governorate. The following results were obtained: a- Five genera were collected and identified in the Mycology section, Botany Dept., Faculty of Science, Assiut University. Fungal colonies were counted per gram in fresh” sample. b- The most common genera were Aspergillus, Penicillium, Rhizopus, and Fusarium. c- Twenty-two isolates of Fusarium species (~. moniliforme, F. solani, ~. eguiseti and ~. oxysporum: B, 10, 3 and 1, respectively) were isolated from the samples and examined for their ability to produce mycotoxins in a liquid medium. Thin layer chrDmatDgraphic analysis revealed that zearalenDl was detected in 8 iSDlates Df F. mDnilifDrme and Dne iSDlate Df ~’ sDlani, while diacetDxyscripenDI was detected in Dne iSDlate Df F.sDlani d- ChrDmatDgraphic analysis Df the sDxhlet extractiDn Df the samples revealed that zearalenDne was fDund in 7 samples, aflatDxin 8 1in 3 samples, aflatDxins 81 and Gl in 4 samples while diacetDxyscripenDl and T-2 tDxin in Dne sample. 11- Review Df the recDrds in the examined farms revealed the fDIIDwing: At EI-Hawateka farm: 1- There was nD clinical deviatiDn frDm nDrmal health Dther than high percentage Df infertility and SDme cases Df abDrtiDn. 2- There was a significant decrease in milk yeild in the winter seaSDn Df 1984 and a highly significant decrease in the winter and spring seaSDns Df 1985 cDmpared with that o f 1983. 3- PDst-mDrtum repDrts revealed inflammatiDn Df the genital Drgans in SDme cases in these periDds (1983-1985) assDciated with general inflammatiDn Df Dther Drgans. 4- ND variatiDn Dccurred in rate Df abDrtiDn thrDugh the 3 years, it remained nearly cDnstant being 3.6%. |