الفهرس 
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Abstract
The in vitro antibacterial activity of propolis, florfenicol and
their combination was evaluated against Aeromonas hydrophila
pathogen. Also , the in vivo efficacy of propolis ,florfenicol and
their combination was investigated on Clarias lazera fish
experimentally infected with A. hydro ph ila , after their
administration at their therapeutic dose levels (10 gm Ikg diet) and
(l0 mg I kg b.wt in feed) for 10 successive days, respectively,
First Experiment
I-In-vitro studies:
A- Sensitivity of A. hvdrophila strain to propolis, florfenicol
and their combination (Disc diffusion method).
In vitro sensitivity test of A. hydrophila strain against
propolis, florfenicol and their combination using agar disc diffusion
method showed no inhibition zone to propolis, a clear zone of
inhibition of (25mm) in diameter to florfenicol and a clear zone of
inhibition of (27mm) in diameter to their combination.
B- Minimum inhibitory concentration (MIC) for propolis
(broth dilution method)
The employed MIC of pro po lis- ethanolic-extract is 80 ug/ml.
Il-In-vivo studies:
One hundred and sixty catfish (Clarias lazera) were classified into
eight equal groups each of20.
Group 1:fish were served as non infected non treated.