الفهرس | Only 14 pages are availabe for public view |
Abstract This study aimed to rapid detection and enumeration of some microbial indicators and pathogens (Staphylococcus aureus, Clostridium perfringens, Listeria monocytogenes, Vibrio cholerae, Salmonella typhi, Escherichia coli O157:H7 and Candida albicans) in four different types of water (surface water form River Nile at Rosetta branch, Groundwater (treated and untreated), Wastewater from El-Rahawy drain and Marine water from Marsa Mattrouh governorate beaches) using chromogenic media. The used chromogenic media were HiCrome Aureus agar (Himedia, India), M-CP agar (Himedia, India), HiCrome Listeria agar (Himedia, India), HiCrome Vibrio agar (Himedia, India), HiCrome Improved Salmonella agar (Himedia, India), HiCrome EC O157:H7 agar (Himedia, India) and HiCrome Candida Differential agar (Himedia, India) for detection of pathogens, respectively. Different types of water were examined for detection and enumeration of these microorganisms and sensitivity, specificity and efficiency of the chromogenic media were calculated by identification of typical colonies with polymerase chain reaction technique (PCR) and by identification of atypical colonies with Biolog technique. The limit of detection of these chromogenic media for low numbers of these pathogens was also studied. Survival of these pathogens in two types of water with two different sterilization methods and with two different initial inocula was also studied. |