الفهرس 
GENERAL INTRODUCTION AND LITERATURE REVIEWOnly 14 pages are availabe for public view
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Abstract
Part I
This part contains a general introduction about the chemical names, structures,
physical properties, pharmacological actions and uses of the studied drugs. It also
contains literature reviews for the reported methods of analysis for the studied drugs in
pharmaceutical dosage forms as well as in biological fluids.
Part II
This part comprises three chapters.
Chapter 1
This chapter describes a simple, reliable and rapid HPLC method for the
simultaneous quantitative determination of ciprofloxacin hydrochloride and
hydrocortisone in pharmaceutical preparations. The chromatographic analysis was
performed on a C18 column. A mobile phase consisting of 55% phosphate buffer
solution and 45% methanol was used. The two compounds were determined
simultaneously using the diode-array and multiple wavelength detector at 278 nm and
246 nm. Under the optimized experimental conditions, the graphs obtained by plotting
the peak areas and the concentrations of ciprofloxacin hydrochloride and hydrocortisone
were found to be linear over the concentration ranges 9.5 – 37.5 μg/ml and 40 – 160
μg/ml respectively. Limits of detection were 0.53 μg/ml and 1.44 μg/ml and limits of
quantitation were 1.78 μg/ml and 4.82 μg/ml for ciprofloxacin hydrochloride and
hydrocortisone respectively. The specificity of the proposed method was ascertained by
monitoring the possible interferences of the mobile phase, the placebo and the
degradation products of the drugs of interest. The results obtained by the proposed
method were statistically validated. The developed method was successfully applied to
the determination of the studied drugs in pharmaceutical preparations.
Chapter 2
This chapter describes a simple, reliable and rapid HPLC method for the
simultaneous quantitative determination of ascorbic acid and rutin in pharmaceutical
preparations. The chromatographic analysis was performed on a C18 column. A mobile
phase consisting of 55% dilute orthophosphoric acid solution and 45% methanol was
used. The two compounds were determined simultaneously at 254 nm. Under the
optimized experimental conditions, the graphs obtained by plotting the peak areas and
the concentrations of ascorbic acid and rutin were found to be linear over the
concentration ranges 50 – 200 μg/ml and 25 – 100 μg/ml respectively. Limits of
detection were 3.07 μg/ml and 1.88 μg/ml and limits of quantitation were 9.29 μg/ml
and 5.69 μg/ml for ascorbic acid and rutin respectively. The specificity of the proposed
method was ascertained by monitoring the possible interferences of the mobile phase,
the placebo and the degradation products of the drugs of interest. The only exception
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was the degradation product of rutin in alkaline medium which had the same retention
time as that of ascorbic acid and was coeluting with ascorbic acid when mixed together.
The results obtained by the proposed method were statistically validated. The developed
method was successfully applied to the determination of the studied drugs in
pharmaceutical preparations.