الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 130 |  |  |
| | | from | 130 | | |
Abstract
ABSTRACT
Ibrahim, Ahmed Abdelfatah.
The Use of Hepatocellular Carcinoma Cell Line and Brine Shrimp Larvae for Detection of Antitumor Activities and Toxicity of Selected Egyptian plants.
M. Sc., Fac. Sci. Ain Shams Univ., Cairo, 2010
Key words Egyptian plants, extraction, Cytotoxic effect, Antitumor, in vitro bioassay, fractionation, human tumor cell lines, Hep G-2, Brine Shrimp Lethality.
The objective of this thesis is to evaluate the cytotoxic / anti-tumor effect of some plant extracts on brine shrimp nauplii and hepatocellular carcinoma cell line.
Thus, one hundred and twenty plant species , were collected randomly from different areas in Egypt. The plant Extracts were then screened through two in-vitro bioassays (brine shrimp lethality and hepatocellular human cell line (HepG2) for investigating their cytotoxic and antitumor activities.
The five most active methanol plant extracts on brine shrimp lethality bioassay were chosen as promising bioactive materials and their LC50 and LC90 values were calculated and then subjected to an initial fractionation scheme. These Plants were Citrus maxima, Dovyalis caffra, Tephrosia purpurea, Agave Lophantha, Phlomis aurea.
The Six most active methanol plant extracts on hepatocellular human cell line (HepG2) bioassay were chosen as promising bioactive materials and their LC50 and LC90 values were calculated and then subjected to an initial fractionation scheme. These Plants were Solanum elaeagnifolium, Dovyalis caffra, Tephrosia purpurea, Agave Lophantha, Herniaria fontanesii, Ononis vaginalis.