الفهرس 
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Abstract
Doaa Ahmed Mohamed Hamza. “Molecular genetic studies on drought
tolerance in wheat”. Unpublished Master of Environmental Science
thesis, Department of Agriculture Science, Institute of environmental
Studies and Research, Ain Shams University, 2011.
Five Egyptian wheat cultivars and seven entries (Triticum aestivum
L.) were used to fingerprint them and estimate the genetic diversity using
RAPD analysis. The five Egyptian wheat cultivars showed high similarity
values among them, where the highest value was between Sakha93 and
Giza168 (0.969) and the lowest one was between Sakha93 and Sakha94
(0.840).The seven entries possessed moderate similarity values among
them, since the highest one was noticed between Line6 and Line7 (0.911)
and the lowest one was scored between Line1 and Line7 (0.585). In
addition, six positive and five negative markers were detected for some of
the used genotypes. The biochemical analysis and enzyme activity assay for
Esterase showed maximum number of scored bands was five bands and
didn’t give a molecular marker related to drought resistance, but generally
the band intensity was high due to the drought treatment. Total Protein gel
electrophoresis showed some bands expressed under drought conditions
only, while others appeared under normal irrigation and a band with a
molecular weight of 162.235 K Da, was expressed under drought conditions
only, as in the Giza168,Line3 and Line4 while in Sakha93 a band with
112.19 KDa was expressed also under drought condition. Also the
expression of the following molecular weight 27.380,17.752, 15.337and
12.564 Kilo Dalton under drought conditions (in Sakha 93, Gemmiza9,Sids6,
Line2 and Line5) respectively, the protein band with low molecular weight
protect the plants from damage to environmental stresses such as drought
and also there are proteins that accumulate with the drought stress in the
plant.
Keywords: Triticum aestivum, Randomly Amplified Polymorphic DNA (RAPD),
Genetic Diversity, Estrease, Total protein gel protein, molecular markers.