الفهرس 
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Abstract
ACV was screened for their inhibitory effect against BHV-1 in-vitro in MDBK cells using virus yield reduction test and recording CPE induced by BHV-1. The antiviral effect of ACV on BHV-1 was in dose dependant manner. ACV at concentrations of about 0.4; 0.5; 0.6; 0.7µg/ml reduced the CPE of BHV-1 by 9%; 17%; 42%; 59%, respectively, in post-infection treatment assay and by 17%; 25%; 50%; 64%, respectively; in simultaneous infection treatment assay while pre infection treatment assay showed that ACV had no effect on progression of CPE of BHV-1. ACV drastically decreased BHV-1 titre(s) in dose dependent manner in both simultaneous and post-infection treatment assays. In simultaneous treatment assay, there was significant decrease in virus titer by 0.35; 0.58; 1.44; 2.34 logs due to concentrations 0.4; 0.5; 0.6; 0.7µg/ml of ACV, respectively. Also, ACV could decrease virus titer by 0.45; 1; 1.29; 2.47 logs, respectively, in post-infection treatment assay, while did not affect the virus titre in pre-infection treatment assay. In conclusion, ACV was able to abolish viral titre and progression of CPE in MDBK cell cultures infected with BHV-1 when being added simultaneously and post-viral infection The antiviral activity of anise oil against BHV-1 was investigated in this study. Post-infection technique gave better results than that obtained in Pre-infection and simultaneous infection techniques with respect to antiviral activity. Concentration of anise oil of about 10 – 100µg/ml were non-toxic to MDBK cells and those concentration were used in this study .The concentration of anise oil viz. 60 µg/ml; 80 µg/ml ; 100µg/ml showed a plaque reduction by 60%; 84%; 99% respectively. Titre(s) of BHV-1 recovered from supernatant of MDBK cells infected with virus and treated with anise oil were greatly influenced reaching almost 4.1 × 103 PFU/ml.