الفهرس 
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Abstract
Objective: Evaluation the antioxidant and hepatoprotective activities of the 80% methanol extract as well as the EtOAc and BuOH fractions of the wild Fennel (Foeniculum vulgare (Subsp; Piperitum) and cultivated Fennel (Foeniculum vulgare var. azoricum). In addition, total phenolic compounds in the 80% methanol extract of Fennel wild and cultivated herbs.
Material and Method: Four hundred gram of air dried powdered herb of wild and cultivated Fennel were sonicated with aqueous mcthanol (80%), successively extracted with Hexane, EtOAc and n-BuOH, the EtOAc and n-BuOH Were subjected to repeated
ilica gel and Sephadex LH-20 column chromatography. The antioxidant activity was measured as the free radical (DPPH). Hcpatoprotective activity was carried out using a waster male rat (250-300 gm). Total phenolic and flavonoids content were dctcrmined as chlorogenic acid and rutin equivalents respectively Results: Two phenolic compounds, i. e. 3, 4-dihydroxy-phenethylalchohol-6-0- caffeoyl-~-D-glucopyranoside and 3’, 8’-binaringenin were isolated from the herb of wild Fennel, their structures were elucidated by spectral methods including lD, 2D NMR and UV. The EtOAc and BuOH fractions of wild Fennel were found to exhibit a radical scavenging activity higher than those of cultivated one. In vitro method of rat hepatocytes monolayer culture was used for the investigation of hepatotoxic effect of the 80% methanol extract of the wild and cultivated Fennel, which were> 1000 and 1000 IlglmL respectively. As well as; their hepatoprotective effect against the toxic effect of paracetamol (25mM) was exerted at 12.5 Ilg/mL concentration. Conclusions: Fennel (Foeniculum Vulgare) is a widespread plant species commonly used as a spice and flavoring agent. The results obtained in the present study indicated that the Fennel (F. vulgare) herb is a potential source of natural antioxidant. Two phenolic compounds, i. e. 3, 4-dihydroxy-phenethylalchohol-6-0- caffeoyl¬~-D-glucopyranoside (A) and 3’, 8’-binaringenin (B) were isolated from Fennel wild herb for the first time.