الفهرس 
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Abstract
The highest laccase production by Pleurotus ostreatus and P. sajor-caju (0.190 and 0.612 U/ml) had been recorded on medium II. Pleurotus sajor-caju produced laccase higher than P. ostreatus on both medium I or II. Also the amount of laccase produced by P. sajor-caju and assayed by ABTS 2,2\- azino ¬bis (3 - ethyl - benzothiazoline - 6 sulfonic acid) (0.468 and 0.612 Vlml) were more than that produced and assayed by OMP 2,6 - dimethoxy phenol (0.357 and 0.467 U/ml) on medium I and II respectively. The results revealed that the highest laccase (1.800 U/ml) was recorded on medium I supplemented with 200 JlM CUS04 and assayed by ABTS after 21 days incubation. Comparing different three buffers used in laccase assay, it was clear that citrate buffer was the best one. Citrate buffer gave laccase (4.917 and 4.270 U/ml) on media I and II respectively. P. sajor-caju could removed 98.0% of 25 mg/L M.B after 7 days and 32.00/0 of 500 mg/L methylene blue after the same period. P. sajor-caju could removed more than 92% of M.B till concentration 150 mg/L, while it could removed 100% of Max. up to 200 mg/L after the same incubation period. The results revealed that as the dose of gamma radiation increased, the growth and laccase production by P. sajor-caju decreased gradually.