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العنوان
Enterobacteriaceae in Sheep and Goat Carcasses in El Gharbia Province =
المؤلف
El-Mayet, Mohamed Gamal El-Dein Ali.
هيئة الاعداد
مشرف / ابراهيم عبد التواب سماحة
مناقش / محمد محمد ابراهيم موسى
مناقش / عبيد عبد العاطى صالح
باحث / محمد جمال الدين على الميت
الموضوع
Meat Hygiene.
تاريخ النشر
2012.
عدد الصفحات
78 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
30/12/2012
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - الرقابه الصحية على اللحوم ومنتجاتها
الفهرس
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Abstract

A total of 100 random samples of retailed meat represented by sheep and goat meat (50 of each) were collected from different butcher’s shops and abattoirs at El Gharbia Province. The samples were examined bacteriologically to evaluate the microbial status of sheep and goat’s carcasses in El Gharbia Province via making total Enterobacteriaceae count and total coliform count and determine the frequency of members of family Enterobacteriaceae in examined sheep and goat samples.
The obtained results revealed that:
• Enterobacteriaceae were detected on 100% of the samples. The mean value of total Enterobacteriaceae counts of sheep and goat meat were 10.42 X 104 ± 5.36 X 104, 3.60 X 104 ± 1.22 X 104 cfu/g, respectively.
• There is a significant difference between the mean values of total Enterobacteriaceae counts of sheep and goat meat (P < 0.05). T-test of total Enterobacteriaceae counts between sheep and goat meat was 5.28 significant at (P < 0.01).
• Coliforms were detected on 100% of the samples.
• The mean value of total Coliform counts of sheep and goat meat were 5.37 X 104 ± 3.09 X 104, 1.11 X 104 ± 3.2 X 103 respectively.
• There is a significant difference between the mean values of total coliform counts of sheep and goat meat (P < 0.05). T-test of total coliform counts between sheep and goat meat was 5.28 significant at (P <0.05).
• It is clear that total Enterobacteriaceae counts in sheep samples were higher than its counts in goat samples.
• Identification of members of Enterobacteriaceae on sheep meat samples revealed the isolation of:
• E. coli and Shigella dispar at the rates of 40 % and 12 %, respectively.
• Shigella Sonni, Shigella dysentery and Enterobacter Aerogenes at the rate of 8 % of each.
• Enterobacter Gergoviae, Citrobacter Diversus, Proteus vulgaris and Salmonella Spp. at the rate of 4 % of each.
• Klebsiella pneumonia, Klebsiella Oxitoca, Proteus Mirabilis and Morganella Morganii at the rate of 2 % of each.
• Identification of members of Enterobacteriaceae on goat’s meat samples revealed the isolation of:
• Shigella dispar, Citrobacter Diversus and Citrobacter Amalonaticus at the rate of 12 % of each.
• Klebsiella Oxitoca and Serratia Marcescens at the rate of 10 % of each.
• E. coli and Enterobacter Aerogenes at the rate of 8 % of each.
• Citrobacter freundii, Hafnia Alvei, Yersinia Pseudotuberculosis and Serratia Liqefaciens at the rate of 4 % of each.
• Shigella Sonni, Shigella Dysentery, Enterobacter Gergoviae, Klebsiella pneumonia, Salmonella Spp. and Serratia Rubidaea at the rate of 2 % of each.
• Salmonella Enteritidis and Salmonella Paratyphi A were isolated from sheep samples at the rate of 2 % of each, while in goat samples at the rate of 2%, 0%, respectively.
• The obtained results also revealed that E. coli was isolated from examined sheep meat samples at ratio of 40 % and the isolated serotypes were O111:K58 and O127:K63 at ratio of 10 %for each, O55:K59 at ratio of 8 %, O157:H7 and O114:K96 at ratio of 6 % for each.
• In addition, the obtained results revealed that E. coli was isolated from examined goat meat samples at ratio of 8 % and the isolated serotypes were O126:K71, O119:K69, O125:K70, O127:K63 at ratio of 2 % for each.
On the basis of the previously mentioned results, Enterobacteriaceae still constitutes common contaminants of meat and meat products and its presence in meat is very important due to its public health significance, good hygienic measures must be applied to obtain safe and wholesome meat.