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Abstract Incorporation of alternative feed ingredients in poultry diets has become a popular strategy to counteract the rising prices of traditional ingredients. Distillers dried grins with solubles (DDGS), the corn eo-product of ethanol industry, becomes one of the common alternative ingredients. Although DDGS may be seen as a cost competitive alternative ingredient, there are concerns regarding its use in poultry diets such as: 1) Overall nutrient variability and lowered amino acid digestibility. Therefore, it would be beneficial to identify feed additives that could improve DDGS utilization in poultry diets. Phytogenics have recently got considerable attentions as feed additives due to their stimulant effects on growth performance and nutrient utilization. 2) Unbalanced electrolytes; alternative feed ingredients (DDGS and canola meal) contains high level of dietary anions (chloride and sulfur), and low level of dietary cations (potassium) which lower the dietary electrolyte balance and induce acidosis that could depress the bird performance, increase litter moisture and/or induce leg problems. However, phytase enzyme was found to be capable of increasing sodium utilization in the gut. Therefore, phytase supplementation to diets containing DDGS, canola meal, and/or supplemental chloride could ameliorate the acidic shift that occurs in the dietary electrolyte balance. The present work was conducted in three experiments as following: Experiment 1 This experiment was conducted at the Poultry Research Unit, Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt to |