الفهرس 
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Abstract
The aim of this study was, achieved through isolation of E. coli from cattle (diarrhoeic and apparently healthy calves) and humans (diarrhoeic and apparently healthy children) as well as detection of shiga toxin producing E. coli by PCR.
This study was carried out on a total of 140 rectal swabs that collected from calves (80 diarrhoeic calves and 60 apparently normal calves) from military farm at Alex. Also, 100 rectal swabs were collected from children (50 diarrhoeic child and 50 apparently healthy child) from governmental hospital in Alex, during the period from January to August 2010.
The samples were examined bacteriologically and the isolates were confirmed biochemically (catalase test, Oxidase test, Indole test, Methyl red test, Voges Proskauer test, citrate utilization test, Urease test).
Also, the polymerase chain reaction tests were used for identification of shiga toxin II gene in the collected samples.
The obtained results were summarized as following:
1- from a total of 140 collected rectal swabs (80 diarrhoeic calves and 60 apparently normal calves), the purified E. coli was isolated from 56 (80 %) and 48 (70%).
2- from a total of 100 rectal swabs (50 diarrhoeic child and 50 apparently normally child). Identified E. coli was isolated from 12 (24%) and 35 (70%).
3- The pathogenic E. coli was detected by hemolysis and congo red binding.
a- from a total of identified E. coli isolated cultivated on blood agar medium, 5 from calves isolates (4.80%) showed -haemolysis phenotype and 99 (95.19%) showed no haemolysis.
b- from a total of identified E. coli isolates cultivated on blood agar medium, 14 from children isolates (29.7%) showed -haemolysis and 33 (70.2%) showed no haemolysis.
c- E. coli from calves isolated binding congo red was 100%, also E. coli from children binding congo red was 100%.
4- Polymerase chain reaction amplification of SLT-II gene for 14 E. coli isolated (7 cattle isolates and 7 human isolates).
a-SLT-II gene was detected in 2 out of 4 diarrhoeic calves samples with a percentage of 50%, also in 2 out of 3 apparently normal calves (66.66%).
b- SLT-II gene was detected in 2 out of 3 diarrhoeic children isolates (66.66%), also in 2 out of 4 apparently normal children isolates with a percentage of 50%.
c- The number of positive isolates for SLT-II from human samples was the same at cattle isolates (57.14%).