الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Baculoviruses are subdivided into two groups depending on the type of
budded virus envelop fusion protein; group I utilized gp64 which include the
most of nucleopolyhedroviruses (NPVs), group II utilized F protein which
include the remnants of NPVs and all Granuloviruses (GVs).
Recent studies reported the viral F protein coding gene as a host
cellular sourced gene and may evolutionary acquired from the host genome
referring to phylogeny analysis of fusion proteins. Thus, it was deduced that
F protein coding gene is species- specific nucleotide sequence related to the
type of the specific host and if virus could infect an unexpected host, the
resulted virus may encode a vary F gene.
In this regard, the present study utilized the mentioned properties of F
gene in an attempt to produce a model of specific and more economic wider
range granulovirus bio- pesticide able to infect both Spodoptera littoralis
and Phthorimaea operculella larvae.
Multiple sequence alignment and phylogeny analysis were performed
on six members of group II baculovirus, novel universal PCR primers were
manually designed from the conserved regions in the alignment graph,
targeted to amplify species- specific sequence entire F gene open reading
frame (ORF) which is useful in molecular identification of baculovirus in
unknown samples. So, the PCR product of SpliGV used to prepare a
specific probe for the F gene of this type of virus.
Results reflected that it is possible to infect S. littoralis larvae by
PhopGV if injected into larval haemocoel, the resulted virus of this infection
showed by using DNA hybridization technique to be encode to F gene
homologous with the F gene of Spli GV, which is revealed that the resulted
virus acquired this F gene sequence from the host genome after infection.
Consequently, these results may infer that if genetic aberrations occur
in the host genome, this may affect in baculoviral infectivity. So, this study
aimed to investigate the effect of gamma radiation at doses around those
used in Lepidopteran male sterilization technique, one of insect control
program, on the susceptibility of the irradiated cells to virus infection.