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العنوان
Phytochemical and Biological Studies of Chorisia
crispiflora, Suaeda maritima and Brachiaria reptans
الناشر
Cair unvirsty
المؤلف
Rasha Refaat Abd El-Latief
هيئة الاعداد
مشرف / M.A. Mansour
مشرف / Farag, Ragaa
مشرف / Ahmad ,M
مشرف / Ragaa M.A. Mansour
تاريخ النشر
2010
عدد الصفحات
225P:.
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Chemistry (miscellaneous)
تاريخ الإجازة
1/1/2010
مكان الإجازة
جامعة القاهرة - كلية العلوم - chemistry
الفهرس
Only 14 pages are availabe for public view

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from 244

Abstract

Chorisia crispiflora H.B. & K., Suaeda maritima (L.) Dumort. and Brachiaria reptans (L.) C.A. Gardner & C.E. Hubb. are three selected species of plants belonging to three different families (Bommbacaceae, Chenopodiaceae and Gramineae), respectively. Chorisia crispiflora H.B. & K. is a Brazilian medicinal plant, used as a folk medicine for rheumatism and menorrhagia. Suaeda maritima (L.) Dumort. is used as traditional medicine for treatment of hepatitis. Brachiaria reptans (L.) C.A. Gardner & C.E. Hubb. has an economic importance as it is a good fodder grass. These medical and economical importances suggest the need for phytochemical and biological studies.
This work included introduction, review of literature on the three studied species which was surveyed with their chemical constituents and biological activities, taxonomy and materials, apparatus and methods.
Results and Discussions included:
A. The preliminary phytochemical screening of the alcoholic extracts of Chorisia crispiflora H.B. & K., Suaeda maritima (L.) Dumort. and Brachiaria reptans (L.) C.A. Gardner & C.E. Hubb. revealed the following:
Presence of flavonoids, carbohydrates and/or glycosides and tannins in all investigated species.
Presence of saponins in Brachiaria reptans only.
Sterols and/or triterpenes were detected in Chorisia crispiflora and Suaeda maritima but absent in Brachiaria reptans.
Absence of alkaloids, anthraquinone and coumarins in all investigated species.
B. Structure elucidation and identification:
The plant material was dried, and extracted by repeated percolation with 70% methanol till exhaustion. The extract filtered and the solvent was removed under reduced pressure and then subjected to column chromatography using water as eluent with increasing concentration of ethanol. The isolated compounds were further purified using PC and sephadex LH-20 column.
Structure elucidation of the pure isolated compounds were carried out through Rf values, colour reaction, chemical analysis (complete and partial acid hydrolysis, enzymatic hydrolysis, hydrogen peroxide oxidation and ferric chloride degradation) as well as spectral analysis (UV, 1H NMR, 13C NMR, HMBC, FABMS, ESIMS and EIMS).
Eighteent compounds (14 flavonoids, 2 phenolic acids and 2 phenolic acid derivatives) were isolated and identified from the three studied species.
Eight from Chorisia crispiflora H.B. & K. and were found to belong to flavones, flavonols and phenolic acids as follows:
Flavones (apigenin 7-O-neohesperidoside; apigenin 7-O-β-glucoside; apigenin 6, 8- di-C-β-glucopyranoside; isovitexin and apigenin).
Flavonols (kaempferol 3, 7-di-O-α-rhamnopyranoside)
Phenolic acids (ellagic acid and gallic acid).
Seven from Suaeda maritima (L.) Dumort. and were found to belong to flavonols and phenolic acid derivatives as follows:
Flavoneols (quercetin 3-O-robinoside-7-O-sophoroside; kaempferol 3-O-robinoside-7-O-sophoroside; kaempferol 3-O-robinoside-7-O-