الفهرس | Only 14 pages are availabe for public view |
Abstract Field samples of cpv-2 were collected and isolated on vero cells. The isolates were identified by fluorescent antibody technique, electron microscopy and peR .The isolates were sequenced with the localy prepared vaccinal strain. Their sequences were compared with those of foreign vaccines traded in Egypt. The % of identities between isolates of group 2 , strain of locally prepared vaccin and strains of foreign vaccines traded in Egypt were 95, 94 and 96 respectively. They were identified as cpv2,cpv2b and 2 respectively. Phylogenetic analysis was done in between isolates, vaccinal strains and reference viruses.Amino acid sequence was done for the isolates and the lovally prepared vicinal strain AA changes were identified. |