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Abstract The live B. melitensis Rev-1 vaccine is considered the best and the most effective vaccinal strain available for prophylaxis and controlling brucellosis in small ruminant. However its administration with a full dose displays a number of drawbacks. In this study, hot saline extract (HSE) and periplasmic soluble Brucella protein (SBP) were extracted from B.abortus S19 and their protective activity against challenge with a field isolate was recovered from aborted ewe was measured in BALB/c mice. Also we assessed their efficacy for stimulation of the humoral immune response of Rev-1 vaccine and their efficacy as subcellular vaccines with/without Montanide -206 adjuvant by in sheep. Heat killed whole cell, SBP and HSE were compared as coating antigens in IELISA. This study concluded that SBP &HSE could provide sheep with satisfactory protection against brucellosis and could also increase the immune response of reduced-reduced dose of Rev-1 vaccine without risk of abortion in pregnant animals. Also SBP could be used as coating antigens in IELISA for diagnosis of brucellosis. |