الفهرس 
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Abstract
In the current study, 7 types of live large animal vaccines that were received by central laboratory for evaluation of veterinary biologics, Abbasia, Cairo over a period of 4 years (2009-2012) were tested for detection of BVDV. One Batch of foetal calf serum and another batch of new born calf serum were tested also for contamination with BVDV as they represent one of the main sources of cell cultures and virus stock contamination and subsequently vaccine contamination.
Enzyme linked immunosorbent assay (ELISA), indirect fluorescent antibody technique (IFAT) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used for detection of BVDV in both different vaccine types and bovine serum batches.
Comparing between the three methods, there was agreement between their results revealing that all the vaccine samples were satisfactory as they were not contaminated with bovine viral diarrhoea virus.