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العنوان
Detection of bovine viral diarrhoea virus in commercial vaccines used in farm animals /
المؤلف
Seioudy, Manar Fathy Hassan,
هيئة الاعداد
باحث / منار فتحى حسن سعودى
مشرف / محمد عبد الحميد شلبي
مشرف / أحمد عبد الغني السنوسي
مشرف / ماجدة محمد سيد
الموضوع
Viral diarrhea. Bovines. Farm animals. Vaccines.
تاريخ النشر
2012.
عدد الصفحات
116 Leaves ;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2012
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Virology
الفهرس
Only 14 pages are availabe for public view

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from 131

Abstract

In the current study, 7 types of live large animal vaccines that were received by central laboratory for evaluation of veterinary biologics, Abbasia, Cairo over a period of 4 years (2009-2012) were tested for detection of BVDV. One Batch of foetal calf serum and another batch of new born calf serum were tested also for contamination with BVDV as they represent one of the main sources of cell cultures and virus stock contamination and subsequently vaccine contamination.
Enzyme linked immunosorbent assay (ELISA), indirect fluorescent antibody technique (IFAT) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used for detection of BVDV in both different vaccine types and bovine serum batches.
Comparing between the three methods, there was agreement between their results revealing that all the vaccine samples were satisfactory as they were not contaminated with bovine viral diarrhoea virus.