الفهرس 
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Abstract
HspA2 is a molecular chaperone that assists in the folding, transport and assembly of proteins in the cytoplasm, mitochondria and endoplasmic reticulum. Hsps have a protective action on the cellular auto-regulation in response to stress and on the mechanism of homeostasis, providing a balance between protein synthesis and degradation. In addition, HspA2 is involved in the regulation of spermatogenesis.
Aim:The present study aimed to investigate the relationship between expression of heat shock protein (HspA2) in ejaculated human sperm and oxidative stress in male infertility.
Patients & Methods: This study included 96 men attending the Andrology Outpatient Clinic, Mansoura University Hospital. The semen samples obtained from men were grouped according to criteria of WHO into: Normozoospermia (N) was used as control group (n=24), Asthenozoospermia (A) (n= 21), Astheno-Teratozoospermia (AT) (n=23) and Oligo-Astheno-Teratozoospermia (OAT) (n=28). Computer assisted semen analysis (Auotosperm), hypo-osmotic swelling (HOS) test and acrosin activity of spermatozoa by gelatinolysis test were performed. Also, malondialdehyde (MDA)/spermatozoa and total antioxidant capacity (TAC) were assessed in seminal plasma. Expression level of HspA2 mRNA of spermatozoa was determined by RT-PCR and DNA fragmentation was detected by agarose gel electrophoresis.
Result: The current study showed that, percentage of DNA fragmentation was significantly increased in OAT group compared to control group (N). Also, the present study showed significantly negative correlation between MDA/spermatozoa with sperm concentration, grade A motility grade A+B motility, velocity, linear velocity linearity index, normal morphology, acrosin activity index, HOS test TAC and HspA2 expression. HspA2 expression and TAC level showed significantly positive correlation with sperm concentration, grade A motility, grade A+B motility, velocity, linear velocity, linearity index, normal morphology, acrosin activity index, HOS test and HspA2 expression.
Conclusion: from results of the current study, it could be concluded that HspA2 gene expression in ejaculated sperm from infertile might be associated with spermatogenic and/or spermiogenic dysfunction involved in the pathogenesis of some cases of male infertility.