الفهرس 
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Abstract
The abundance and diversity of Avian Influenza Viruses in live poultry markets (LPMs) have been recognized since the 1970s, and AIV are recognized as key to the emergence of pandemic Influenza. More recently, there has been increasing recognition of their role in the amplification and maintenance of AIV, in introduction of infection to poultry farms, and in zoonotic transmission of AIV to humans. Therefore the present study was done on live birds sold in private poultry shops in live bird markets.
A total of fifty (50) fresh broiler chicken carcasses were collected randomly from different privet poultry shops at Damanhour city. they examined bacteriologically and Virogically and results reveled that:-
1- Total Enterobacteriaceae count in poultry meat were ranged from 40 X 103 to 10 X 105 with a mean value of 32 X 104 ± 3.45 X 103 cfu/g,
2- Total Mesophilic bacterial count in poultry meat ranged from 15 X 103 to 86 X 104 with a mean value of 27 X 104 ± 30 X 103 cfu/g
3- Total Psychrotrophic bacterial count in poultry meat ranged from 20 X 103to74 X 104 with a mean value of of 23 X 104± 27 X 103 cfu/g
4- declares that salmonella strains could be isolated from examined poultry meat samples was identified as salmonella pullorum strain 5(10%).
5- E.coli could be isolated as 4(8%) from examined poultry meat samples and also Escherichia blatte 4(8%), Enterobacter gergaviae 4(8%), Citrobacter amalonaucus 1(2%), Citrobacter diversus 1(2%), , Cedecea lapageidavisae 3(6%), Klebsiella oxytoca 4(8%) , Klebsiella ascorbata 5(10%), Klebsiella rhinoscleromatis 3(6%), Proteus mirabllis 6(12%), Serratia flarie 5(10%), Shigella 3(6%),, Kluyvera cryocrescens 1(2%), ,Edwardsiella tarda4(8%) and Xenorhabdus luminescens 3(6%) could be isolated
6- Incidence of Coagulase positive Staph. aureus is 63.6 %.
Virolological examination revealed that.
Tracheal and cloacal swabs were collected from broiler chickens at Damanhour city ( the sample from the same chickens that bacteriologically examined and the swabs was collected before slaughtering with taking three birds from each shop and tracheal and cloacal swab from each bird then make polling for these sex swabs together ). Using RT-PCR test in detection of AI in the examined samples reveled that from 50 samples there was one sample positive AI, using specific primers targeting H5,H7 and H9 subtypes, the sample was positive H9 subtype. This result indicate that
*AI could be isolated from LPMS with possibility of isolation of another subtypes including HPAI subtypes H5 and H7.
* possibility of transmission to humans dealing with live birds in live poultry shops.
*possibility of spreading to poultry farms.
So strict hygienic measures should be taken in consideration for prevention and control on AI.