الفهرس 
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Abstract
Bovine tuberculosis, caused by infection with Mycobacterium bovis,
continues to be a major economic problem in several countries and
constitutes a serious public health risk in a number of developing
countries.
The principal aim for control of bovine tuberculosis is to eliminate the
risk of infecting humans, and in developed countries, the aim of
vaccination of cattle should be ideally to prevent the establishment of
infection. This is a difficult challenge as the aim of vaccination
programmes against most human and animal pathogens is to prevent
clinical disease, rather than infection. Since vaccination of cattle is
unlikely to be totally effective, ancillary diagnostic testing of cattle would
have to be undertaken in association with vaccination and vaccinated
animals should not react in the tuberculin or alternative diagnostic tests.
in our study we aimed to evaluate the vaccine of bovine tuberculosis
(BCG) and its ability to prevent confusing between vaccinated animals
and infected animals at using of bovine tuberculosis diagnostic tests.
In our study we tested 900 animals (420 cattle and 480 buffaloes) from
different localities at Minufiya and Gharbia governorates .By application
of tuberculin test on these animals, it showed that 9 cattle (2.14 %) and 27
buffaloes (5.62 %) were positive tuberculin reactors while the remaining
animals were negative. from these negative reactor animals we
vaccinated 6 animals with Bacilli Calmette Guerin (BCG), and retesting
of these vaccinated animals with tuberculin test has been done after 8
weeks and gave negative tuberculin reaction .Positive tuberculin tested
animals were slaughtered and examined to determine the post mortem
lesions which were 8 (88.9%) out of 9 cattles and 4(14.8%) out of 27
buffaloes showed visible lesions.
Summary
98
The lymph nodes and tissue samples were collected from the positive
tuberculin (9) cattles and (27) buffaloes.
On histopathology on the positive reactor tissues by Z.N. staining of acid
fast bacilli, we found that all positive cattles 9(100%) gave positive when
stained with Z.N. While 5 (18.5%) tissues gave positive with Z.N from
27 positive tuberculin buffaloes. and 17(62.96%) tissues gave positive
with H&E.
On the bacteriological examination of the tuberculin positive cattle
tissues which were (8 with VL and 1 with NVL), 8 (88.9%) acid fast
bacilli were isolated. The total 8 tissues showed visible lesions grew on
Lowenstein-Jensen medium with sodium pyruvate and all the 8 isolates
(100%) gave positive with ZN stain. In buffaloes, the total acid fast
bacilli isolated from 27 tuberculin positive buffaloes were 17 (62.9%) .9
colonies grew on L.J with glycerol and 8 grew on L.J with sodium
pyrovate . With examination by ZN stain, all 17 isolates (100%) gave
positive results
After using PCR and LCD hybridization array indicated that higher
prevalence of Mycobacterium tuberculosis complex in all cattle cases(9)
and that confirmed by the genotyping using MIRU technique that
detected M.bovis typical types on the contrary expressed by buffalo cases
especially after the results of PCR and LCD hybridization array that gave
Mycobacterium other than tuberculosis (MOTT) and other than the
typable species in the list of the array test this means atypical types .
We used ELISA with 4 types of antigens (PPD, ESAT-6, CFP10,
MBP70) to discriminate between negative, infected and vaccinated
animals .This test showed that all positive tuberculin reactor animals gave.