الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 147 |  |  |
| | | from | 147 | | |
Abstract
H. pylori is a spiral-shaped bacterium, it is one of the most common causes of gastritis and peptic ulcer diseases. It is known to colonize in the gastric mucosa causing chronic gastritis and intestinal type of gastric carcinoma and MALT-lymphoma.
There are many researches that confirm the relationship between the H. pylori and diseases of the digestive tract such as stomach ulcers and duodenal ulcers some studies have also demonstrated the presence of this microbe in extragastric disorders manifestations includes: rosacea, glaucoma, chronic rhinosinusitis and OME. Also, H. pylori contribute to GER and laryngeal carcinogenesis. It has been noted a marked improvement in the symptoms of these diseases are listed after the elimination of this microbe with antibiotics.
Therefore, the objective of this research is to detect and genotype this microbe in common laryngeal pathologies.
Fifty five cases were included in this study attending the Otorhinolaryngology Department for laryngeal surgery and samples were taken from them, while they were suffering from: hoarseness, throat infections, minimal associated pathological lesions of the larynx (MAPL’s), laryngeal carcinoma, excluded patients in this study who were given antibiotics before the operation.
In our results, H. pylori was positive in 3/20 patients (15.0%) of laryngeal carcinoma, in polyps 2/15 patients (13.3%), in nodules 5/15 patients (33.3%), and in keratosis 2/5 patients (40%). Totally 12/55 patients (21.8%) were positive for H. pylori in laryngeal pathology.
In the present work, it was found that in all of the cases, which were H. pylori positive in the larynx and in the lower esophageal sphincter, had two genotypes: Genotype I = (CagA -ve & VacA -ve & IceA1 -ve & IceA2 +ve) were found in 3 (15.0%) patients, 2 (13.33%) patients and 5 (33.33%) patients for laryngeal carcinoma, polyps and nodules, respectively. Genotype II = (CagA -ve & VacA +ve (s2/m1) (inactive toxin / greater gastric epithelial damage) & IceA1 -ve & IceA2 +ve) were found in 2 (40.0%) patients of keratosis only.
Genotyping of H-pylori +ve were identical in laryngeal lesion and lower esophageal sphincter, that they indicated came from the same source, and most probably came from the stomach.
where the predominant genotypes in the 55 biopsies in the present study that, were positive for H. pylori by PCR in the vocal folds lesion was the iceA2 (22%) and iceA1 (zero%).