الفهرس 
INTRODUCTION & AIM OF WORKOnly 14 pages are availabe for public view
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Abstract
The present study was designed to investigate some toxicological
effect of the bromadialone as anticoagulant rodenticides which used to
control the rodents that caused great economic losses to growing, animal’s
farms and fabric of buildings .Beside the rodent are involved in the
transmission and dissemination of many parasites and diseases to man and
his domestic animals.
Therefore, the present study aimed to investigate the harmful side
effect of bromadialone which used on earth and plant so it may be inter the
food cycle of human and cause a great harmful effects.
The present study investigated two items:-
1- mutagenic effect of anti coagulant rodenticide bromadialone, on the
bone marrow cells( chromosomes aberrations assays) of the maternally
treated newborn and their mothers with different doses of bromadialone
(1/10 LD50 of bromadialone ;0.1125 mg/Kg b.w.&1/20 LD50of
bromadialone ;0.0562 mg/kg b.w. on day 15 only and on day 8 and15) of
pregnancy , classical methods of cytogenetic was carried out to study the
chromosomal abnormality. .
2- Investigation histopathological effect of the anticoagulant rodenticide
bromadialone on (Liver, Kidneys and Lungs) of the maternally treated
newborn and their mothers with different doses of bromadialone (1/10 LD50
of bromadialone ;0.1125 mg/Kg b.w.&1/20 LD50of bromadialone ;0.0562
mg/kg b.w. on day 15 only and on day 8 and15) of pregnancy .
The used rats in the present work were divided in different groups:
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173
Group(1):
The control animals were of the same inbred strain of the treated
animals.
Group (2):
pregnant mothers were treated orally with one dose (1/10 LD50)
(0.1125 mg/kg b.w.)of bromadialone at the day 15 of pregnancy .
Group (3):
pregnant mothers were treated orally with two doses (1/10 LD50)
(0.1125 mg/kg b.w.)of bromadialone at the day 8 &15 of pregnancy .
Group(4):
pregnant mothers were treated orally with one dose (1/20 LD50)(0.0562
mg /kgs b.w.)of bromadialone at the day 15 of pregnancy .
Group (5):
pregnant mothers were treated orally with two doses (1/20 LD50)
(0.0562 mg /kgs b.w.)of bromadialone at the day 8 &15 of pregnancy .
Mothers and early postnatal newborn directly were collected for the
examination of chromosomal aberration and for the histopathological
investigation(liver, kidney and lungs).
The present investigation includes the following items; structural
aberrations , numerical aberrations, mitotic index and histopathological
study.
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174
6-1 structural aberrations:-
In the case of treatment of mother’s rat with two different doses of
bromadialone at day 8 and day 15 of pregnancy, deletion, centromeric
attenuation and gap appear in high rate.
In the case of treatment of mothers rat with one dose of
bromadialone in day 15 of pregnancy, deletion, centromeric attenuation
appear in high rate.
6-2 numerical aberrations:-
Numerical aberration appear as (monosomic ) in bone marrow of
treated mother and their new born.
6-3 Mitoyic index:-
Rate of mitotic index decrease in the new born and their mother treated with
different doses of bromadialone.
6-4Histopathological study:
The mothers and their newborns at delivery treated with
bromadialone showed histopathological alternation in the liver, kidneys
and lungs.
LIVER :
Regarding the histopathological effects of bromadialone
rodenticide on the liver of albino rat, Rattus norvegicus (newborns and their
mothers after oral administration with different doses of bromadialone
(1/10LD50 of bromadialon(0.1125mg/Kg b.w.), 1/20 LD50 of
bromadialone(0.0562mg/kg b.w.) on day 15 only and days( 8 &15 )of
pregnancy showed , different shapes of histopathological changes i.e ,
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175
necrosis ,lymphatic infiltration, fatty change ,hydropic degeneration and
venous congestion.
Kidney:-
The kidney of maternally treated newborn and their mothers of
albino rats, Rattus norvegicus after oral administration 1/10 LD50 of
bromadialone; 0.1125 mg/kg b.wt&1/20 LD50 of bromadialone; 0.0562
mg/kg b.wt , respectively on day 15 and days 8&15 showed degenerative
effects , i.e. necrosis of the epithelial cells lining the convoluted proximal &
distal and collecting tubules of the cortex , focal infiltration atrophied
glomeruli; cloudy swelling , hydropic degeneration and glomerulonephritis
of the malpighian corpuscles , haemorrhage in the medullary portion and
congestion blood vessels
Lung:-
The lungs of the maternally treated newborn and their mothers
showed some histopathological changes ;pulmonary oedema appears as a
homogenous faintly stained oesinophilic fluid in the terminal and respiratory
bronchioles and alveolar ducts.As well as there is peribronchiolar lymphoid
cell agg.