الفهرس 
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Abstract
A total number of 668 apparently healthy fish were obtained from farm to study the effect of two heavy metals (Copper and Mercury) on some hematological, biochemical parameters of blood, immunology, histopathological alteration in kidney, liver, spleen, muscles and gills and the residues in fish muscles. The LC so /96 hr. of Cu and Hg were estimated and fish exposed to Yz LCso for 7 days and for 1/10 LCso for 8 weeks from each product separately.Results showed decrease in RBCs count ,PCV% and Hb in acute and chronic mercury while a
significant increase was shown in acute and chronic copper toxicity , total leucocytic count showed decrease in acute mercury toxicity and increase in the chronic case, while in copper
toxicity non significant decrease in acute and significant decrease in chronic toxicity was noticed. Elevated serum urea and creatinine in both acute and chronic mercury and copper toxicity was detected. No changes in total bilirubin in the acute mercury and chronic copper toxicity while significant increase in chronic mercury and acute copper .Elevation of serum AST and AL T in some days of acute toxicity of mercury and copper while in chronic mercury toxicity a significant elevation of both serums AST and ALT were detected .while in chronic copper toxicity serum AST was fluctuated and AL T showed no significant changes.CK study revealed significant decrease in acute mercury with fluctuation in the chronic toxicity while in copper toxicity it showed fluctuation in acute and significant decrease in chronic toxicity. Glucose value decreased in acute and chronic mercury toxicity while in copper toxicity it showed significant increase in the acute and increase followed by significant
decrease in the chronic copper toxicity. Total protein showed no significance changes in both mercury and copper toxicity except only the decrease that detected in chronic mercury toxicity. Results of estimating MW of serum protein by SDS-PAGE in both acute and chronic
mercury and copper toxicity showed presence of specific bands with different MW does not
appear in the control group. MTT in mercury and copper toxicity revealed significant decrease in the viability of stimulated cells and un stimulated cells in some days of acute toxicity while in chronic toxicity it induced decrease in the viability of stimulated cells and non stimulated cells. Lysozyme activity showed significant decrease in acute mercury and copper as well as in chronic mercury toxicity while no change was detected in chronic copper toxicity.Cytotoxicty assay reveled significant decrease in both acute and chronic mercury and copper toxicity. Histopathological finding in acute and chronic mercury and copper toxicity revealed detectable changes in different organs. Mercury and copper residues obtained from these studies in the acute and chronic toxicity showered different values of residues in the muscles.