الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 63 |  |  |
| | | from | 63 | | |
Abstract
In order to clarify the possible effect of melatonin hormone on immune system, the present study was carried out to
study the effect of oral administration of melatonin on some immune parameters in adult male rats.
The study was carried out at the Department of Physiology, Faculty of Veterinary Medicine, Zagazig University
Thirty immature male male rats were divided into 3 equal groups. Group (I) maintained as control, Group (11) was subjected to fasting for one day and melatonin was supplemented at the end
fasting and continued for 15 days, Group (Ill) was subjected to
fasting for 2 days and the melatonin was supplemented (orally by
stomach tube at dose of 0.01 mg/lOO g.b.w) at the end of 2°d day
of fasting. The samples were taken after complete course of
melatonin supplementation of 15 days. Samples from control
animals were taken at the same time of taking samples from
group 11 and Ill. Melatonin was supplementation between 1700
slaughtering directly from jugular vein into clean, heparinized tubes and divided into three parts, I St part for total and differential leucocytic count, 2”d part was used for lymphocyte
proliferation assay test and the 3rd part was centrifuged at ’3000
rpm for 20 minutes and plasma was collected after centrifugation
for assays of interleukin-2 and immunoglobulins. Tissue samples
of spleen, thymus were taken and fixed in 10% formalin for histopathological examination.