الفهرس 
Review of literatureOnly 14 pages are availabe for public view
 |  | from | 168 |  |  |
| | | from | 168 | | |
Abstract
The present study aimed to prepare different formulae of oil adjuvant vaccines from killed local isolated Corynebacterium pseudotuberculosis field strain and evaluation of their potency in Guinea-pigs and sheep.
The experimental results revealed that:
1- Out of 200 sheep, lymph nodes with caseous lymphadenitis like lesions, thirteen isolates of Corynebacterium pseudotuberculosis were isolated and confirmed by microscopical examination; CAMP test; biochemical identification and detection and quantitative measurement of PLD by electrophoresis and immunoblot.
2- It was found that the concentrated amount of PLD toxin did not affect by both concentration method, although Millipore ultra-filtration was a practically suitable for vaccine production.
3- It was found that complete inactivation of Corynebacterium pseudotuberculosis using 0.01%, 0.02% and 0.03% BEI was achieved after 4 days, 2 days and 1 day respectively.
4- The cell mediated immune response in case of the two groups of Guinea pigs vaccinated with Corynebacterium pseudotuberculosis vaccine adjuvanted with paraffin oil were less than those observed in the two groups vaccinated with the vaccine adjuvanted with Montanide ISA-206.
5- The humoral immune response of vaccinated Guinea pigs was detected by ELISA showed that the highest mean levels of anti-PLD.
IgG were found in animals vaccinated with Bacterin + toxoid adjvanted with Montanide ISA 206 adjuvant.
6- Mean level of anti-PLD-IgG in animals immunized with formalin killed bacteria was nearly similar to those of BEI killed bacteria immunized Guinea pigs.
7- The mean optical density of the control group in both immunological assay ELTSA and lymphocyte proliferation test were significantly different to those of the immunized vaccinated four groups, confirming that all of the prepared vaccines assessed a significant level of cellular and humoral immunity against Corynebacterium pseudotiiberculosis infection.
8- PM findings in Guinea pigs vaccinated groups revealed that formalized bacterin + PLD toxoid and adjuvanted with Montanide ISA 206 was the best formula to be used for sheep vaccination.
9- Four different vaccines formulae were investigated for their efficiency in protecting sheep against Corynebacterium pseudotiiberculosis. These vaccines formulae were applied in four sheep groups:
Group-1: Vaccinated with 0.4ml of BCG containing 1-2 million organism injected intradennally 2 months pre-vaccination with 2inl containing 20 mg bacterin + 50 ug toxoid + Montanide ISA 206 oil injected S/C as the first dose, then boostered with the same dose 15 days apart.
Group-2: Vaccinated with BCG (1-2 million organism) intradennally using a single dose, simultaneously with the first dose of 2ml
containing 50 g toxoid + Montanide ISA 206 oil adjuvant injected S/C then boostered with the same dose 15 days apart.
Group-3: Vaccinated with 1st dose of 2ml containing 20 mg bacterin + 50g toxoid + Montanide ISA 206 oil adjuvant and boostered with the same dose 15 days apart.
Group-4: Vaccinated with 1st dose of 2ml containing 10 mg bacterin + 50g toxoid + Montanide ISA 206 oil adjuvant injected S/C and boostered 15 days apart (half the dose of bacterin in previous groups).
10- Evaluation of post vaccinal humoral immune response using ELISA and cellular immune response using lymphocyte proliferation assay was performed and statistical analysis indicated that the four vaccines formulae assessed a significant level of cellular and humoral immunity.
11- Four weeks after booster vaccinal dose all sheep groups were challenged with virulent Corynebacterium psendotuberculosis12- Five months post challenge, the protection capacity of each vaccine formulation was evaluated by post mortem examination. The different vaccinated sheep groups showed protection percentages against challenge ranged from 78%-71.2% while unvaccinated challenged sheep showed a protection ratio of 9%.