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Abstract Chronic otitis media has been one of the most important subjects of otologic studies for hundred years. There have been numerous investigations concerning the structure of the tympanic membrane with the light microscope. The submicroscopic observation of this m embrane provides a deeper insight system of keratinizing cellular components and the toward the better understanding of the complicated nature and the arrangement of fibers which could play an important role in the formation of cholesteatoma. ’l’en cadeveric temporal bones have been studied in this work for the observation of the characteristics of the normal tympanic membrane. The ulutrastructure details of the human tympanic membrane have been studied utilizing a Three layers are distinctly recognized, outer epidermal, middle lamina propria and inner mucous layer. In pars flaccida, the middle lamina propria |