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Abstract This study was carried out to study the role of fish in transmission of some microorganism to human. A total of 187 samples (102 fish and 85 human) were aseptically collected from different fish markets in Dakahlia Governorate. Fish samples including; Tilapia niloticus (18), Mugil cephalus (18), Mackerel (18), Sardine (18), Slated Sardine (15) and Smoked Herring (15). Human samples including; hand swabs (30) and stool from apparently healthy fish-seller and stool from patients with gastrointestinal disturbance (20 adults and 15 children).The samples were bacteriological examined for the prevalence of Staphylococcus species, Aeromonas species and Vibrio species. The result showed Staphylococcus species were isolated from 41.52% of total samples including, 64.70% of fish surface swabs, 7.84% of fish homogenate, 90% of fish-seller hand swabs, 65% of fish-seller stool, 1.33% of children stool and 20% of adults stool. Aeromonas species were isolated from 15.22% of total samples including, 17.64 of fish surface swabs, 16.66 of fish homogenate, 20% of fish-seller hand swabs, 6.66% of children stool and 15% adults stool. Vibrio species were isolated from 43.25% of total samples including, 50% of fish surface swabs, 52.94% of fish homogenate, 60% of fish-seller hand swabs and 10% of adults stool. On the other hand, Aeromonas and Vibrio species were not isolated from fish-seller stool (control). Also Vibrio species were not isolated from children stool. |