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Abstract In the present study, two medicinal plants (S. reflexcum and A. stipularis) were investigated for its organic constitution and biological activity with the object of discovering new chemical components of expected biological activity. Through this investigation, the plant material was extracted and purified by chromatographic techniques, such as. SEPARO medium-pressure liquid chromatography (MPLC), column chromatography (CC) (silica gel, Sephadex LH-20, reversed phase C-18) and thin layer chromatography (TLC) (analytical and preparative). The isolated compounds were characterized by spectroscopic techniques (1H NMR, 13C NMR,1H-1H COSY, TOCSY, NOESY, HMBC, HSQCdept, DEPT, MS and GC–MS), physical data as well as chemical transformations, and the results were analyzed and compared with the literature data. Pharmacological tests; in vitro anti-cancer and antischistosomiasis tests, were used to test the activity of the extracts. Chemical constituents of S. reflexcum (Asteraceae) The petroleum ether extract of S. reflexcum was fractionated by accelerating gradient chromatography (AGC) with gradient elution by petroleum ether then with mixtures of petroleum ether, methylene chloride and methanol. Eluted fractions were evaluated by TLC and combined upon similar appearance, yielding seven fractions. The fraction no 6 was eluted from the column with gradient elution running from hexane to CH2Cl2 and MeOH in increasing polarity to afford α-epi-lupeol [155] (37 mg). The methanol extract of S. reflexcum was subjected to GC-MS analysis and 4 compounds were characterized as: myristic acid ester ENGLISH SUMMARY 13 [156], oleic acid ester [157], palmitic acid ester [158] and stearic acid ester [159]. |