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Abstract Comparative tuberculin test was applied and result was: 75% in cattle and 65.6% in buffaloes. ELISA test results revealed 21.25% were +ve from cattle and 12.5% from tested buffaloes. Totally 38/192 representing 19.7%. Isolation resulted in 10 isolates representing 26.3% (9 isolates from cattle and 1 isolate from buffaloes) all were fecal not tissue culture. Molecular identification on the isolates by LCD array revealed that the isolates belonged to Mycobacterium.avium complex , by PCR 4 isolates were positive (3 isolates from cattle and 1 buffalo isolate). |