الفهرس 
BMC Systems BiologyOnly 14 pages are availabe for public view
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Abstract
The 16S rRNA genes (rDNA) of 50 strains belonging to 26 different coryneforrn bacterial
species and genomospecies and of the type strain of Rhodococcus equi were
enzymatically amplified. Amplified rDNA restriction analysis (ARDRA) with the enzymes
AJul, Cfol and Rsal was carried out. The combination of the ARDRA patterns obtained
after restriction with these three different enzymes enabled the differentiation between
the following species: Corynebacterium accolens (number of strains = 2), C. afermentans
subsp, afermentans (2), C. afermentans subsp, lipophilum (2), C. amyco/atum (3),
CDC corynefocm group ANF-l-like (1), CDC coryneform group ANF-3-1ike (1), C. cystWd/
s (1), C. d/phL~/ae (4), C. jeikeium (3), C. macginleyi (2), C. minutissimum (1), C. pilosum
(1), C. pseudotuberculosis (2), C. rena/e (2}0 C. striatum (2), C. urealyticum (3), C.
xeros/s (1), CDC coryneform groups I1-1 (2), B-3 (2), F-l, genomospecies 1 and 2 (6), G,
genomospecies 1 (1) and G, genomospecies 2 (2).
The following strains or species could not be differentiated from each other: C. pseudodiphtheriticum
(2) from C. propinquum (former CDC coryneform group ANF-3) (2),
CDC coryneform group F-l, genomospecies 1 (4) from genomospecies 2 (2) and C. jeikeium
genomospeci~s A (I) from genomospecies C (2).
ARDRA may represent a possible alternative for identification of coryneforms, since
this technique enabled the identification of most coryneforms tested and since DNA
extraction (i.e. cell i/sis by boiling), amplification, restriction and electrophoresis can be
carried out within 8 hours. This might allow quick identification of C. diphtheriae and
other possible pathogens of the genus Corynebacterium.