الفهرس 
Historical backgroundOnly 14 pages are availabe for public view
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Abstract
Helicobacter pylori is one of the most common causes of gastritis and peptic ulcer diseases. In addition, it causes adenocarcinoma of the stomach and mucosa associated lymphoid tissue lymphoma (MALToma).
There are several methods available for detecting H. pylori infection including:
1) invasive methods based on gastric biopsies
2) non invasive methods like Urea Breath Test (UBT), serology and stool antigen tests.
3) PCR technique.
While there appears to be little doubt that H.pylori can be detected in the oral cavity by PCR, one may still question the importance of this finding. This is due to:
First, PCR allows for the detection of low numbers of bacteria, which may be too few to influence gastric health.
Moreover, PCR can permit detection of non viable H.pylori, which is, by definition, non infectious.
We aimed to study the value of salivary helicobacter pylori detection by PCR to differentiate between functional dyspepsia and acid-ulcer syndrome.
We conduct this study on 30 cases suffering from dyspepsia divided into three groups according to histopathological examination of gastric biopsy.
Group 1: confirmed to H.pylori positive and suffering from ulcers or erosions.
Group 2: confirmed to H.pylori positive without ulcers or erosions.
Group 3: proved to be H.pylori negative.
Using rapid urease test we found that:
10 cases (100%) of group 1 were positive (with ulcers or erosions).
10 cases (100%) of group 2 were positive (without ulcers or erosions).
10 cases(100%) of group 3 were negative.
This gives the test 100% sensitivity and specificity respectively.
Using PCR assay of salivary samples,
Helicobacter pylori were detected in:
10 cases (100%) of group 1, with mean PCR value = 948300.
7 cases (70%) of group 2, with mean PCR value = 1378800.
3 cases (30%) of group 3, with mean PCR value = 144500.
• This gives the test sensitivity and specificity 80% and 90% respectively.
• ROC curve for the best cut off point to differentiate between H.pylori positive and negative patients was at value of 500000.
• Using 500000 PCR value as cut off point can also differentiate between two groups of patients :
• Group of patients with functional dyspepsia with their PCR value less than 500000, and group of patients diagnosed as gastritis up to gastric ulcer with their PCR values more than 500000. But still we can not differentiate between gastritis and gastric ulcer patients subgroups using their salivary PCR values.