الفهرس 
Liver physiologyOnly 14 pages are availabe for public view
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Abstract
Stem cell therapy holds great promise for the repair of injured tissues and organs, including the liver.
The aim of the present study was to demonstrate the effect of transplanted haematopoietic stem cells on liver functions in experimental acute liver failure in rats.
Thirty adult male albino rats, weighing 150-200 gram, were used in this work.
The rats were classified into three groups; the first group was the normal control group and the second group was acute liver failure-non-treated group in which rats were undergone acute liver failure by single I.P injection of acetaminophen in a dose of (1g/kg). Finally, the third group was acute liver failure-stem cell- treated group in which acute liver failure rats were treated with single intravenous injection of stem cells (in a dose of 2x 106cells/rat).
At the end of the experiment (after 4weeks) in each of the previously mentioned groups, retro-orbital blood samples were used to estimate serum ALT, AST, bilirubin, albumin and PT.
The rats were sacrificed and livers were excised for histopathological and immunohistochemical studies.
The results showed that induction of acute liver failure by acetaminophen was associated with significant increase of serum ALT, AST, bilirubin, and PT, but the serum albumin was not affected when compared with the normal control group.
Histopathological examination revealed significant increase of the percentages of affection of hepatocytes.
Also H&E stained sections from the livers showing degenerative and necrotic changes in hepatocytes.
These changes were marked in the centrilobular areas where there was marked necrosis, disorganization and loss of hepatocytes in areas adjacent to the central vein.
The necrotic changes were either in the form of pyknotic or-106 -Summary karyolitic nuclei, in addition to cytoplasmic changes, or complete loss of the cells with distortion of the hepatocyte plate architecture.
PAS-stained sections of acute liver failure-non-treated group depletion of glycogen granules from the hepatocytes.
In Masson’s trichrome stained sections of acute liver failure-non-treated group an increase in the greenish stained collagen fibers was observed in between the hepatocytes.
Immunohistochemical stained sections from acute liver failure- non- treated group showed negative staining for the monoclonal mouse anti-human hepatocytes.
In the present investigation single intravenous injection of hematopoietic stem cells was associated with insignificant decrease in the mean value of serum ALT, AST and albumin and significant decrease in the mean value of serum bilirubin and PT when compared with the corresponding values in acute liver failure-non-treated group.
Histopathological examination revealed significant decrease of the percentages of affection of hepatocytes.
H&E stained sections from acute liver failure-stem cell-treated group showed a picture nearly similar to the normal control group liver.
PAS- stained sections of acute liver failure-stem cell- treated group showed more or less a normal amount of glycogen granules of hepatocytes.
Sections stained with Masson’s trichrome technique showed scanty greenish colored collagen fibers in between the hepatocytes,a picture nearly similar to normal control group.
Immunohistochemical stained sections, from this group showed a positive brownish staining for the monoclonal mouse anti-human hepatocytes.
The staining appeared granular in the cytoplasm and was observed in some of hepatocytes.