الفهرس 
Acute Myeloid LeukemiaOnly 14 pages are availabe for public view
 |  | from | 141 |  |  |
| | | from | 141 | | |
Abstract
Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by a block in differentiation of hematopoiesis, resulting in growth of a clonal population of neoplastic cells or blasts. According to morphology (FAB) group divided AML into 8 subtypes designated M0 through M7).
Moreover, (WHO) classification for AML added the genetic, immunophenotypic, biological and clinical features to the morphological findings.
Flow cytometric immunophenotyping remains an indispensable tool for the diagnosis, classification, staging, and monitoring of acute myeloid leukemia.
CD36 antigen belongs to the class B scavenger receptor family,and was subse¬quently identified as a receptor for matrix protein thrombospondin 1 (TSP-1).CD36 has been implicated in multiple biological processes owing to its characteristic property as a multiligand scavenger receptor that recognizes remarkably diverse molecules.
The present study aimed to assess the expression of CD36 in patient with acute myeloid leukemia, and to evaluate its role in distinguishing AML cases with monocytic lineage.
The current study was carried out on newly diagnosed 40 AML patients who were classified into two groups: Non- monocytic group: included 25 patients (1M0, 9 M1, 8 M2, 7 M3), Monocytic group: included 15 patients (9 M4 and 6 M5); They were subjected to complete history taking, clinical examination, CBC, BM aspiration and flowcytometric immunophenotyping. CD36 expression was detected by using CD36 mouse MoAbs by flowcytometry.
Expression of CD36 was heterogenous in different FAB subtypes. The highest CD36 expression was observed in M5,M4 and followed by M2, while the lowest CD36 expression was observed in M0 and M3. This heterogeneity of CD36 expression, as regards the FAB subtypes, was found to be significant.
CD36 was positively expressed in all cases of the monocytic group, and was positive in only 4 cases of the non-monocytic group. And on comparing monocytic group versus non-monocytic group regarding number of cases positively expressed CD36; it showed a high statistically significant increase in expression of the marker among monocytic group.
CD36 expression was found to have a higher mean with monocytic group versus non-monocytic group and that was statistically highly significant
However, AML FAB M5 was found to have the highest mean among other subtypes including M4.
A high significant correlation was found when comparing CD36 expression with CD14. Also a high significant difference and correlation was found in CD14 expression in monocytic group versus non-monocytic group and among FAB subtypes.
A highly significant correlation was found when comparing CD36 with CD117 and CD34.
On comparing CD36 expression with the expression of CD33, CD13 and HLA-DR, no significant correlation could be found.CD36 expression was not associated with any the studied demographic, clinical or laboratory variables, except for peripheral blast. No statistically significant associations were elicited between CD36 expression and any of the studied prognostic factors of patients except for hepatomegaly.