الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Adipose derived mesenchymal stem cells (ADMSCS) have been continuously come
into focus of interest as an easyaccessible source for pluripotent cell. To date the
use of modified well defined canine ADMSCs (cADM5Cs) in regenerative veterinary medicine has been limited. This is attributed to a missing defined characterization concept of pluripotent marker gene expression. A total of 22 subcutaneous fat samples (approximately 0.S-2 gm wt/sarnple), were collected from the upper regions of limbs as well as ventral abdomen regions of 20 dogs (21.8 ± 13.8 months) old under aid of general inhalation anaesthesia with isoflurane gas. These dogs were
underwent different surgeries without any other evident disease. Herein, we present isolation and characterization of un-diferentiated cADMSCs by a marker set for gene and protein expression confirming the pluripotentcharacteristics of the cells by quantitive RT-PCR (RT-qPCR), conventional RT-PCR and immunocytochemistry. These samples were subjected for isolation and cultivation of cADMSCs. Cultivated
cADMSCs showed spindle fibroblst-like morphology. Positive gene expression of
OCT4, SOX2, KLF4, cMYC and NANOG was confirmed by qPCR. Additionally,
cADMSCs showed positive expression of MSC - specific markers C090 and COlDS, as well as embryonic stem cell -specific surface markers (stage-specific embryonic antigens SSEA-3 and SSEA-4), while the expression of the hematopoietic marker eD4S was lacking and the expression of CD34 was slightly positive. The complete chondrogenic and adipogenic differentiation of cAOMSCs were proven using alcian blue and Oil red 0 staining respectively. Theosteogenic differentiation ability of ADMSCs w~,~.sqn(irmed by positive von Kossa staining as well as positive expression of osteocalcin in the extra cellular matrix after 2 weeks of cultivation in inductive
medium. The demonstrated results allow for future investigations of marker gene and protein characteristics of cADMSCs e.g. in context with gene therapeutic modifications or external protein application of the cells.