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Abstract Nosocomial infection particularly those caused by multiresistant microorganisms that are difficult to treat are one of the main causes of concern for those working in infectious diseases. They are also an excellent indicator of the quality of hospital care. Staphylococci are members of low G-C gram positive bacteria with approximately 30 to 38% G+C content Silvestri and Hill (1965). Staphylococcus aureus is Gram positive, cluster forming coccus, yellow colony on routine agar media, non-motile, non-spore forming, facultative anaerobe, ferments glucose and produces mainly lactic acid, ferments of mannitol (distinguishes from S. epidermidis), catalase positive, coagulase positive and DNas positive. Moreover it is golden hardy microbes as it withstand moist heat at 60 °C for 30 minutes, grows in media containing 7.5% to 10% Sodium chloride, remain viable for months on agar plates stored at 4°C, and grow at 6.5°C to 40C ( Kloos & Bannerman, 1995 and Baird, 1996).Staphylococci are an important cause of human diseases which vary in severity from skin and soft tissue infections to necrotizing pneumonia. Staphylococcus aureus is the most common cause of nosocomial infections.MRSA has emerged worldwide as an important nosocomial pathogen since the 1970s over the years, widely introduced and standardized permitting accurate detection of MRSA and VRSA strains from clinical specimens. In the present study about 900 cultures from clinical samples taken (Urine , Sputum and pus from wounds) from patients in critical care patients and from surrounding environment in ICUs and CCU of critical care department of Cairo university teaching hospitals and detect some genes responsible of the phenomenon of resistance ,the results of isolation from patients and admission places. |