الفهرس | Only 14 pages are availabe for public view |
Abstract In this work some bacteriological and immunological studies on Nigella sativa (N. sativa) were carried out as for evaluation of the N. saliva antibacterial and immunomodulatory effects on the immune cells, specifically monocyte. Blood samples were collected from healthy chickens for separation of peripheral blood mononuclear cells. At first, one hundred twenty native breed (baladi) chickens of average weight about 700 gm/chicken were used in the study. The chickens were divided in to two experimental groups. The first group contained sixty chickens and received black seed oil.. The second group also contained sixty chickens and left as control. Chickens in both groups feeded with regular maintenance ration. Blood and serum were collected from each group at (2, 4 and 6 weeks) post receiving the black seed oil. So, at the beginning culturing of peripheral blood mononuclear cells and this method has described a simple reproducible method for obtaining a high yield viable monocytes. The immunomodulatory effect of the black seed oil group have the typical morphologic same the control group. |