الفهرس 
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Abstract
The aim of investigation was applied to detect and
characterize virulence factors among Aeromonas species
isolated from diseased Oreochromis niloticus by using
different methods specially DNA methods.
Two hundred of diseased fish were subjected to full
clinical, post- mortem and bacteriological examinations.
The clinical signs of diseased fish were noticed such
as haemorrhagic septicaemia all over the body of fish
specialy at the end of fms, fin rot, scale loss, skin ulcers,
abdominal ascitis, corneal opacity in one or two eyes,
inflammations at the end of vent and all the internal organs
were found to be congested.
from the bacteriological investigation for naturally
infected fish, the following bacteria were isolated A.
hyDROPhila, A. sobria, A. caviae, Pseudomonas group and
Enterobacter group.
In this study, the total prevalence of Aeromonas spp.
infection among the examined Oreochromis niloticus was
65%. A. hyDROPhila was the highest incidence (40 %)
followed by A. sobria (17.5 %) and A. ea viae (7.5%) which
differentiated between them by some bacteriological
examinations such as M. R. and some sugars as esculine,
arabinose and salicine.
Nine Aeromonas spp. isolates (3 A. hyDROPhila, 3 A.
sobria and 3 A. caviae) were randomly selected and tested