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Abstract Pestban is broad spectrum organophosphate insecticide with growing concern due to its aquatic toxicity especially freshwater fish as (Orechromis niloticus) locally called Nile tilapia is an important popular fish species in Egypt and is abundant in the natural water source. Green barley has many antioxidants such as antioxidant vitamins, superoxidismutase, catalyze enzyme and chlorophyll which chelate and adsorb toxic materials as pesticide. This work was planned to investigate: I-Determination of the median lethal concentration of Pestban which cause death to 50% of tilapia fish (45±5g, I2.5±0.5cm) at 96-h.This experiment used 180 tilapia fish divided into ten concentrations (0.07: 0.6mg/l) of Pestban. 2- Study protective effect of barley grass powder (10% and 20%in diet) on toxic effects of pestban this experiment used 540 tilapia fish divided into 4 groups as follows: GroupI: control (-ve) basal diet. GroupII: (control +ve) barley grass powder 10% |