الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The monitoring of such drugs is important for quality assurance in preparations and for obtaining optimum therapeutic concentrations in body fluids to minimize the risk of toxicity. However, in the past decade, only limited studies have been performed on major and minor tranqulizers. Therefore, in the first part of this study, it is important to develop simple and sensitive spectrophotometric methods for the determination of chlorpromazine HCl (CPZ), thioridazine HCl (THZ), bromazepam (BZ) and diazepam (DZ). Four new, simple, rapid and accurate spectrophotometric methods have been developed for the analysis of the psychoactive drugs CPZ and THZ in dosage forms and in biological fluids. The methods are based on the oxidation of CPZ and THZ by a known excess of N- bromosuccinimide and KMnO4 under acidic conditions followed by the determination of unreacted oxidants by four different reaction schemes. Three simple, accurate, sensitive and economical spectrophotometric methods are presented for the determination of BZ and DZ drugs in both pharmaceutical preparations and urine. The proposed extractive spectrophotometric methods are based on formation of yellow ion-pair complexes between BZ and DZ drugs and three different sulphophthalein dyes such as bromocresol green (BCG), bromophenol blue (BPB) and bromocresol purple (BCP) in acidic buffer solution. The formed complexes were extracted with methylene chloride and measured at the selected wavelength. The overall methods have been successfully applied to assay of the four drugs in pharmaceuticals, serum and urine samples.
For toxicological studies; hundred and twelve mature albino rats of both sex (males and females) weighting 140 to 170 gm were used. Seven rats from each sex intraperitoneally administered (1, 1, 5 and 5 mg/kg B. wt) BZ, DZ, CPZ and THZ once daily for one month, seven rats from each sex served as control. Results revealed significant increase in the level of the enzyme activities of both superoxide dismutase (SOD) and catalase and significant decrease in luteinizing hormone (LH) and follicular stimulating hormone (FSH) in both treated groups in most cases. Residual detection of CPZ, DZ and BZ are carried out using Gas chromatography with flame ionization detector (GLC–FID) demonstrated presence of drugs residues in the examined samples. Histopathological findings of liver, brain and the reproductive organs proved the previously mentioned results.