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العنوان
Genetic and Molecular Studies on Some Agronomic Traits in Faba Bean/
المؤلف
MOUSA, DINA SOLIMAN MOSSELHY.
هيئة الاعداد
باحث / دينا سليمان مصيلحى
مشرف / عصمت عبد العظيم والى
مناقش / ابو المعارف محمد ضمرانى
مناقش / محمد حسام ابو النصر
الموضوع
beans.
تاريخ النشر
2011.
عدد الصفحات
103ص. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البساتين
الناشر
تاريخ الإجازة
28/3/2011
مكان الإجازة
جامعة أسيوط - كلية الزراعة - البساتين(خضر)
الفهرس
Only 14 pages are availabe for public view

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from 121

Abstract

Two investigations were carried out at the experimental farm of faculty of Agriculture, Assiut University, during the winter seasons of 2007/2008 and 2008/2009. The investigations were carried out to study the inheritance of some continuously variable characteristics of faba bean as well as study the performance of five faba bean genotypes.
The inheritance studies were carried out using diallel cross analysis among some genotypes of faba bean. The characters studied showed the existence of important genetic variation in all cases. Significant G.C.A. and S.C.A. effects for all the characters were observed. Regarding to general combining ability effects (gi) for the parents, which compare the average performance of each parent with other parents and facilitate selection on parents for initiation of breeding population for subsequent improvement, in Experiment A, line Assiut 125 is a good general combiner for early flowering, large number of tillers per plant, large number of dry pods per node, large number of dry pods per plant, high weight of dry seed per plant, high percent of hard seed and low height of first fruiting node. Line Romy 12 is a good general combiner for height of first fruiting node, large number of seeds per pod, large green pod and high percent of hydration coefficient and protein content. Also, Line Assiut 104/2, is a good general combiner for early flowering and plant height. Therefore, these parents could be excellent parents in breeding programs.
For individual cross combination, the cross Romy 13 x Assiut 104/2 showed a high performance for early flowering, large number of dry seeds per pod and low height of first fruiting node. the cross Romy 12 x Assiut 104/2, showed a high performance of large number of tillers per plant, large number of dry pods per plant and high weight of dry seed per plant.
Also, the performance of five faba bean genotypes were studied. Assiut 125 gave the highest yield in both seasons, while Assiut 104/2 and Romy 3 gave the lowest yield in both seasons, respectively. In addition, Assiut 125 was significantly better than all other lines in number of dry pods per plant and dry seed weight/plant.
Seven RAPD markers were used to detect the genetic variability and relationships among five faba bean lines. The results of RAPD analysis showed that six out of the seven tested primers displayed polymorphism among the all tested lines, while one primers were monomorphic. 47.83%out of 69 bands amplified were polymorphic. Unique DNA fragments with different sizes were detected in particular lines but not in the others using different primers. Unique DNA markers with different sizes were related to some traits such as green pods/plats, weight of dry seeds/plant, tellers/plant and protein content percentage traits The genetic similarity among the five faba bean lines was high, ranging from 96.7 % to 76 %. The highest similarity and shortest genetic distance were found between Romy 80 and Romy 3. While the lowest genetic similarity and longest genetic distance were found between Romy 80 and Ass.85/37. The investigation suggested that the RAPD approach showed considerable potential for identifying and discriminating faba bean line.