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Abstract
Isolation of E. coli from mastitic udder and feaces : The economic importance of Bovine mastitis can be summarized as an indicative measure of dairy farm profitability , fertility of the cows, length of calving interval and the general parameters for good economic results because it was correlated with different aspects of gross margin of dairy farms. Also, it is used for selecting cows for treatment, identify animals that should be culled and identify healthy cows for purchasing
Mastitis caused by E.coli was more likely to be sever and during recent years , bovine coliform mastitis was found to become very common .It continued to be major problem and reflected the poor hygienic conditions. The sever clinical effect of coliform mastitis has been attributed to endotoxin absorbed from the stimulated release of cellular product.
So this work was planned to detect the similarity of E.coli serotypes in bovine mastitis milk and feces .
To achieve that, the following was carried out:
1- Isolation of E.coli from mastitic milk and fecal samples.
2- Biochemical identification of the recoverd isolates.
3- Serological identification of E.coli that were isolated from mastitic cases and fecal samples .
4- Studying virulence factors of E.coli isolates that were recovered from mastitic buffaloes, cows and fecal samples including Congo red binding activity , haemolytic activity.
This study was carried-out on a total number of 110 lactating cows and buffaloes from different villages and farms in Menofia Governorate were investigated for cilnical and subclinical mastitis .
The samples collected were , 250 indivdual quarter milk samples and 110 fecal samples collected from mastitic cases .
Collection of milk samples .
California mastitis test .
The results of this study indicated that:
1- There is a significant differences for prevalance of clinical and subclinical mastitis incidence and percentage among examined cows and buffaloes .As, the incidence of clinical mastitis was 37 quarters from the total examined quarters with a percentage of 8.4 %. Meanwhile the number of quarters showed subclinical mastitis was 213 quarters with a percentage of 48.4 %.
2-The distribution of Positive quarters among mastitic animals (Cows,Buffaloes)
1- Showed significant differences among cows and buffaloes in its distribution and the incidence of the number of quarters infected with mastitis. The higher incidence of mastitis in cows higher than that of buffaloes.
2- In cows the incidence of mastitis in four quarter constituted the higher incidence level, followed by one quarters, then three quarters and the least level is the incidence of mastitis in two quarters.
3- In buffaloes the higher incidence of mastitis in four quarters constituted the higher level, followed by three quarters, then two quarters and one quarters.
4- The number of positive quarters to E. coli showed higher level of incidence in cows (9.2 %) than that of buffaloes (2.3 %).
5- The results also showed that, showed significant incidence of the mastitis scoring among different milk samples examined.
The Score+ ++ showed the higher level 117 samples with a percentage (26.59.9 %) , followed by Score++ about 70 sample with a percentage (15.9 %) and the lower score is subclinical mastitis score + of 63 sample with a percentage of ( 14.3 %).
6- The results also, showed that from 250 milk samples, the incidence of clinical mastitis about 8 samples from the 37 sample examined with a percentage 21.6 %. While the incidence of clinical mastitis about 5 samples from 213 samples examined with a percentage of 2.3 %.
7- The results also indicated that the E. coli isolates from milk constituted in O146, O168 and those isolated and from rectal swab include, O158, O26, O78, O114 and O78.