الفهرس 
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Abstract
In this study we report the validation and establishment of a real time
PCR assay using a TaqMan labeld probe for the detection of
Mycoplasma ga/lisepticum. The MG rtPCR assay had the same
specificity and was 10 folds more sensitive than cPCR. The detection
limit of MG rtPCR was 1.6 CFU/ml, while it was 16 CFU/ml in cPCR.
A total of 71 unvaccinated poultry flocks were examined, from which
16 (22.5%), 24 (33.85), 34 (47.9%) and 38 (53.5%) were positive by
culture, ELlSA, cPCR and rtPCR respectively. from the 5 flocks
vaccinated with killed MG vaccine, ELlSA showed positive results for
2 flocks, while the culture, cPCR and rtPCR results were negative for
all of the 5 flocks. However, cPCR and rtPCR showed positive results
for all of the 5 flocks vaccinated with live MG vaccine. While, only 3
flocks were positive by ELlSA and 4 flocks were positive by culture. In
conclusion, the MG rtPCR was highly specific and sensitive than
classical methods for detection of MG.