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العنوان
Identification of salmonella in camel feces by microbiological and molecular assays /
المؤلف
khoulosy, Sherein Galal Mohamed.
هيئة الاعداد
باحث / شيرين جلال محمد خلوصي
مشرف / صلاح الدين عبد الكريم سليم
مشرف / ايھاب محمد ابراھيم
مشرف / سھير شكري الياس
الموضوع
Salmonella. Identification. microbiologie. Camel.
تاريخ النشر
2010.
عدد الصفحات
129 Leaves :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2010
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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from 168

Abstract

Bacteriological examination of 1135 fecal swabs collected from imported and local breed camels to detect Salmonella SPI? The swabs showed that 31 swabs harbored various Salmonella microorganisms with an overall incidence of 2.73 %. The isolates were identified by cultural and biochemical methods. Serotyping of the identified isolates was done. A total of 31 Salmonella isolates (8 Salmonella serovars) were isolated from examined fecal swabs of diseased and apparently healthy camels .It was also noticed that the most recurred serovars were S. Typhimurium and S. Enteritidis which were isolated from fecal swabs collected from both imported and local breed camels. S. Blukwa III a and S. Aarhus serovars were recorded only in fecal swabs collected from imported camels. S. Cerro, S. Haouaria, S. Paratyphi Band S. Kedougou 11 servoars were recorded only in fecal swabs collected from local breed camels . Virulence - associated region in total DNA of Salmonella was detected using PCR . The results of PCR indicated the presence of the SPVC gene associated with virulence only in one serovar, S. Enteritidis. In this study a comparison between the results of Standard Microbiological Technique (SMT) and PCR in detection of Salmonella spp. was performed by direct examination of camel’s fecal swabs.