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Abstract Infectious Bronchitis disease still occurred in vaccinated and unvaccinated chicken’s farms in Egypt. Four variants isolates of IBV could be isolated and identified. One variant strain of them produced respiratory distress and nephritis nephrosis associated with 46.7% mortality compared with the reference variant strain which produced 33.3% mortality, also the variant isolated strains were identified using RT - peR and sequencing of apart of S 1 gene .An inactivated oil emulsion vaccine where prepared from both isolated variant & reference variant strains. it could be proposed that revision of vaccination programs and type of vaccines used should be implemented either as local or imported production. |