الفهرس | Only 14 pages are availabe for public view |
Abstract In this study, LPS antigens were prepared from Brucella abortus strain 99 and Brucella melitensis strain 16 M also outer membrane proteins were prepared from the same strains and ELlSA was applied to evaluate its serodiagnostic efficacy. Several serological testes were applied on different serum samples collected from the field including RBT, Rivanol test, TAT and BAPAT to compare the results in between them and with the prepared LPS and OMPs antigens. So, the present investigation was conducted in 4phasesr Phase I: Obtaining the following standard Brucella strains(B.abortus strain 99 and B. melitensis strain 16M) and identification of these strains by bacteriological examination and antigenic characterization. Phase 11:Separation& Characterization of Outer membrane protein (OMP) of the collected standard Brucella strains_separation using SOS-PAGE and bands of different molecular weights were recorded and the result was discussed. Phase Ill: Preparation of Brucella Lipopolysaccharide Antigen from B. abortus strain99 &8. melitensis strain 16M. |