الفهرس 
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Abstract
The overall prevcalence of Giardia in water buffalo calves was 11.5 % and 12.8 % by direct wet and floatation concentration technique respectively. The morphology of Giardia trophozoites and cysts was examined by Mallory’s haematoxyline stain, and the ultrastructure by TEM. Giardia trophotoites were successefully cultivated in axenic culture (TYI-S-33). SDS-PAGE of Giardia cyst showed 5 polypeptide bands ranging from 112.19 to 21.27 KDa, while 12 bands were reported in Giardia trophozoites antigen ranging from 243.62 to 7.27 KDa. DMA was extracted from Giardia species cysts. PCR method was employed to amplify and sequence a 293 fragment of the small subunit ribosomal RNA (16S- rRNA) gene. Our isolate showed little degree of divergence in sequences from other previous studies with similarity 96.6%. PCR and DMA sequencing showed the species under investigation is Giardia duodenalis.