الفهرس 
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Abstract
Bovine ephemeral fever (BEF) is a noncontiguous epizootic arthropod-borne viral disease of cattle and water buffaloes, the disease is known also as Three-day sickness, Bovine epizootic fever, Three-day stiff sickness, Dragon boat disease, The economic impact of BEF include high morbidity, economic losses in terms of significant reduction in milk production, disruption of national and international trade and finally a variety of complications resulting from the disease (Walker, 2005), transmission of the virus and spread depends largely on the insect vector population, BEFV is classified as the type species of the genus Ephemerovirus several outbreaks have been recorded in Egypt during, 1999,2000,2001,2003,2005, So the present study is designed for characterization of one of the Egyptian isolate of BEFV on the molecular level to put it on the universal Phylogenetic tree to determine the relationship between that Egyptian isolate and BEFV strains from different localities in trying to identify its antigenic diversity, it was found that there is high identity of the local isolate with the Taiwanese and the Japanese isolates on both nucleotide and amino acid level than the Australian cluster so the current study is designed in order to provide an accurate data about the antigenic diversity of the local isolate from other isolates and strains all over the world this data could be valuable to be taken in consideration when designing a vaccination program to control the disease in Egypt at the same time the present study will provide database depending on sequence analysis that may help for future studies, it was also found that the sequenced fragment covering the neutralizing epitope G3a and G3b is conserved in all isolates and the Egyptian isolate EGY-2005as well, so the monoclonal antibodies detecting these sites could be valuable to detect BEFV, it was also found that there is no significant difference between the local isolate and the vaccinal strain 919 in both dot-Immuno blot and western blot techniques and PCR as well.