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العنوان
Biological Activity of Serum and Follicular Fluid Inhibin of Camel :
المؤلف
Mohamed, Faten Aboaziza.
هيئة الاعداد
باحث / فاتن أبو عزيزة محمد
مشرف / عبد القادر أحمد زكى
مشرف / نادية أحمد طه
مشرف / ماجدة قلادة ميخائيل
الموضوع
Serum. Fluid. Camel.
تاريخ النشر
2007.
عدد الصفحات
252 Leaves :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
الناشر
تاريخ الإجازة
1/1/2007
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Physiology
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present work was planned to evaluate and differentiate between the
immunological and biological activity of inhibin in the serum, follicular and
lDIoiotic fluids of pregnant and non pregnant she-camel during breeding and non
breeding seasons. Samples were grouped into (S, S.Mg, S.Ma, S.M.L, S.L, Mg.S,
Ma.S, M.S.L, L.S and L.S.M). Ovine testicular lymph protein fraction IV (OTLP4)
and steroid free bovine follicular fluid were collected and separated. Levels of
immunoreactive inhibin and FSH were determined using indirect ELISA. Also
quaotitative determination of 17 fJ - estradiol using competitive ELISA was done.
The immunological assay was confirmed and differentiated by in vitro and ill vivo
experimental biological assay. The results revealed that there are different types of
the same sized follicle and consequently different inhibin concentrations were
obtained. There was a difference in immunoreactive and bioactive inhibin and the
presence of medium sized follicles (either growing or atretic) with its contents of
inhibin and estrogen control up dominance or down regression. In pregnant she-
camel inhibin levels increase in follicular fluid, serum and amniotic fluid with the
advancement of pregnancy which may be attributed to another sources as corpus
luteum, placenta or fetal gonads.